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Plasmids

Published plasmids are avalible at Addgene and MolecularCloud. For the latest unpublished sensors for ACh, DA, 5-HT, NE, eCB, Ado, ATP etc, please contact yulonglilab2018(a)gmail.com for information.

Drosophila

Transgenic flies carrying published ACh sensor and dopamine sensor are available at Bloomington Drosophila Stock Center.

Genotype Cat#
ACh sensors
w[*]; P{w[+mC]=UAS-GRAB(ACh2.0)}2/In(2LR)Gla, wg[Gla-1] PPO1[Bc] 80046
w[*]; P{y[+t7.7] w[+mC]=10XUAS-GRAB(ACh3.0)}attP40 86549
w[*]; PBac{y[+mDint2] w[+mC]=10XUAS-GRAB(ACh3.0)}VK00005/TM6B, Tb[1] 86550
w[*]; P{y[+t7.7] w[+mC]=10XlexAop-GRAB(ACh3.0)}attP40 86551
w[*]; PBac{y[+mDint2] w[+mC]=10XlexAop-GRAB(ACh3.0)}VK00005/TM2 86552
DA sensors
w[*]; P{w[+mC]=UAS-GRAB(DA1m)}2 80047
w[*]; P{w[+mC]=UAS-GRAB(DA1m)}3/TM2 80048
y[1] w[*]; P{y[+t7.7] w[+mC]=lexAop-GRAB(DA1m)}attP40/CyO 80049
y[1] w[*]; PBac{y[+mDint2] w[+mC]=lexAop-GRAB(DA1m)}VK00005 80050
w[*]; P{w[+mC]=UAS-DA1m-mut}attP40/CyO 80051
w[*]; P{w[+mC]=10XUAS-GRAB(DA2m)}attP40/CyO 90878
w[*]; P{y[+t7.7] w[+mC]=10XlexAop-GRAB(DA2m)}attP40/CyO 90879
w[*]; PBac{y[+mDint2] w[+mC]=10XlexAop-GRAB(DA2m)}VK00005/TM2 90880
5-HT sensors
w[*]; P{y[+t7.7] w[+mC]=10XUAS-GRAB(5-HT1.0)}attP40 90874
w[*]; PBac{y[+mDint2] w[+mC]=10XUAS-GRAB(5-HT1.0)}VK00005 90875
w[*]; P{y[+t7.7] w[+mC]=10XlexAop-GRAB(5-HT1.0)}attP40/CyO 90876
w[*]; PBac{y[+mDint2] w[+mC]=10XlexAop-GRAB(5-HT1.0)}VK00005 90877
PARIS
w[*]; P{y[+t7.7] w[+mC]=QUAS-pHluorin.CAAX}attP40 82174
w[*]; P{y[+t7.7] w[+mC]=QUAS-pHluorin.CAAX}attP2 82175
w[*]; P{y[+t7.7] w[+mC]=10XUAS-pHluorin.CAAX}attP2 82176
w[*]; P{y[+t7.7] w[+mC]=10XUAS-ArchT-P2A-mRuby3.CAAX}attP40/CyO 82177
w[*]; P{y[+t7.7] w[+mC]=QUAS-ArchT-P2A-mRuby3.CAAX}attP40 82178
w[*]; M{w[+mC]=QUAS-ArchT-P2A-mRuby3.CAAX}ZH-86Fb 82179

Viruses

Vigene (China)

AAV viruses carrying published and unpublished GRAB sensors (including the lastest ACh, DA, NE, 5-HT, Ado, ATP, VIP, CCK and eCB sensors) are available here. Note: please contact the Li Lab (yulonglilab2018@gmail.com) before ordering unpublished sensors.

BrainVTA (China)

Virus Cat#
AAV-hSyn-GACh2.0 PT-1309
AAV-EF1α-DIO-GACh2.0 PT-1310
AAV-hSyn-DA1m PT-1298
AAV-EF1α-DIO-DA1m PT-1299
AAV-hSyn-DA1h PT-1301
AAV-EF1α-DIO-DA1h PT-1302
AAV-hSyn-DA mut PT-1304
AAV-EF1α-DIO-DA mut PT-1305

Addgene

Virus Cat#
pAAV-hSyn-GRAB_DA1m 113049-AAV9
pAAV-hSyn-GRAB_DA1h 113050-AAV9
pAAV-hSyn-GRAB_NE1m 123308-AAV9
pAAV-hSyn-GRAB_NE1h 123309-AAV9
pAAV-hSyn-GRAB_DA1h 113050-AAVrg
pAAV-hSyn-GRAB_NE1m 123308-AAVrg
pAAV-hSyn-GRAB_NE1h 123309-AAVrg
pAAV-hSyn-GRAB_NEmut 123310-AAVrg

For international users, please contact yulonglilab2018(a)gmail.com for information.